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1x mem non-essential amino acids solution (neaa)  (Thermo Fisher)


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    Thermo Fisher 1x mem non-essential amino acids solution (neaa)
    1x Mem Non Essential Amino Acids Solution (Neaa), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non-essential+amino+acid+solution+(neaa)/1%C3%97+mem+non+essential+amino+acids/pm40550888-195-22-35
    Average 90 stars, based on 1 article reviews
    1x mem non-essential amino acids solution (neaa) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Infection with Borrelia burgdorferi Increases the Replication and Dissemination of Coinfecting Powassan Virus in Ixodes scapularis Ticks.
    Article Snippet: The media used for the growth of these cells consisted of Minimum Essential Media (MEM, Gibco, Grand Island, NY, USA) with 8% heat-inactivated fetal bovine serum (FBS, Thermo Scientific, South Logan, UT, USA), 1% non-essential amino acid solution (NEAA, Gibco, Manassas, VA, USA), and 1% penicillinstreptomycin antibiotic solution (Gibco, Manassas, VA, USA).

    Article Title: Functional intestinal monolayers from organoids derived from human iPS cells for drug discovery research.
    Article Snippet: Briefly, for the induction of pre-enterocyte-like cells, the intestinal progenitor cells, which were induced for 7 days differentiation, were cultured for 10 days in intestinal final differentiation medium, DMEM high-glucose medium containing 10% KnockOut Serum Replacement (KSR; Thermo Fisher Scientific), 1% non-essential amino acid solution (NEAA; Thermo Fisher Scientific), P/S, 0.5 × B27 supplement minus vitamin A and 1 × GlutaMAX supplement, supplemented with 2 μM SB431542 (SB; FUJIFILM Wako Pure Chemical Industries), 3 nM LY2090314 (LY; MedChem Express), 100 nM 1α,25dihydroxyvitamin D3 (VD3; Cayman Chemical), and 50 ng/mL epidermal growth factor (EGF; R&D Systems).

    Article Title: Functional intestinal monolayers from organoids derived from human iPS cells for drug discovery research
    Article Snippet: Briefly, for the induction of pre-enterocyte-like cells, the intestinal progenitor cells, which were induced for 7 days differentiation, were cultured for 10 days in intestinal final differentiation medium, DMEM high-glucose medium containing 10% KnockOut Serum Replacement (KSR; Thermo Fisher Scientific), 1% non-essential amino acid solution (NEAA; Thermo Fisher Scientific), P/S, 0.5 × B27 supplement minus vitamin A and 1 × GlutaMAX supplement, supplemented with 2 μM SB431542 (SB; FUJIFILM Wako Pure Chemical Industries), 3 nM LY2090314 (LY; MedChem Express), 100 nM 1α,25-dihydroxyvitamin D3 (VD3; Cayman Chemical), and 50 ng/mL epidermal growth factor (EGF; R&D Systems).

    Knock-Out:

    Article Title: Infection with Borrelia burgdorferi Increases the Replication and Dissemination of Coinfecting Powassan Virus in Ixodes scapularis Ticks.
    Article Snippet: The media used for the growth of these cells consisted of Minimum Essential Media (MEM, Gibco, Grand Island, NY, USA) with 8% heat-inactivated fetal bovine serum (FBS, Thermo Scientific, South Logan, UT, USA), 1% non-essential amino acid solution (NEAA, Gibco, Manassas, VA, USA), and 1% penicillinstreptomycin antibiotic solution (Gibco, Manassas, VA, USA).

    Article Title: Functional intestinal monolayers from organoids derived from human iPS cells for drug discovery research.
    Article Snippet: Briefly, for the induction of pre-enterocyte-like cells, the intestinal progenitor cells, which were induced for 7 days differentiation, were cultured for 10 days in intestinal final differentiation medium, DMEM high-glucose medium containing 10% KnockOut Serum Replacement (KSR; Thermo Fisher Scientific), 1% non-essential amino acid solution (NEAA; Thermo Fisher Scientific), P/S, 0.5 × B27 supplement minus vitamin A and 1 × GlutaMAX supplement, supplemented with 2 μM SB431542 (SB; FUJIFILM Wako Pure Chemical Industries), 3 nM LY2090314 (LY; MedChem Express), 100 nM 1α,25dihydroxyvitamin D3 (VD3; Cayman Chemical), and 50 ng/mL epidermal growth factor (EGF; R&D Systems).

    Article Title: Functional intestinal monolayers from organoids derived from human iPS cells for drug discovery research
    Article Snippet: Briefly, for the induction of pre-enterocyte-like cells, the intestinal progenitor cells, which were induced for 7 days differentiation, were cultured for 10 days in intestinal final differentiation medium, DMEM high-glucose medium containing 10% KnockOut Serum Replacement (KSR; Thermo Fisher Scientific), 1% non-essential amino acid solution (NEAA; Thermo Fisher Scientific), P/S, 0.5 × B27 supplement minus vitamin A and 1 × GlutaMAX supplement, supplemented with 2 μM SB431542 (SB; FUJIFILM Wako Pure Chemical Industries), 3 nM LY2090314 (LY; MedChem Express), 100 nM 1α,25-dihydroxyvitamin D3 (VD3; Cayman Chemical), and 50 ng/mL epidermal growth factor (EGF; R&D Systems).



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    GLS1-mediated glutamine metabolism maintains cell proliferation by providing proliferative response substrates. ( A ) Representative bright-field images of liver organoids before (upper panel) and after (lowing panel) the treatment with or without CB839 and <t>NEAA</t> for 48 h (Bar = 1000 μm) and the quantification of organoid size ( B ). ( C ) Cell viability measurement was performed using presto blue. Relative cell viability was calculated by normalizing to Control after 48 h of incubation ( n = 5). ( D ) Protein synthesis in liver organoids treated with or without CB839 and NEAA for 48 h was determined by L-homopropargylglycine (HPG) mean fluorescence intensity (MFI), ( n = 5). (E) Proliferation of liver organoids treated with or without CB839 and NEAA for 3 h was determined by BrdU incorporation, ( n = 5). All data are means ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, one-way ANOVA was used
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    Thermo Fisher non-essential amino acid solution (neaa)
    GLS1-mediated glutamine metabolism maintains cell proliferation by providing proliferative response substrates. ( A ) Representative bright-field images of liver organoids before (upper panel) and after (lowing panel) the treatment with or without CB839 and <t>NEAA</t> for 48 h (Bar = 1000 μm) and the quantification of organoid size ( B ). ( C ) Cell viability measurement was performed using presto blue. Relative cell viability was calculated by normalizing to Control after 48 h of incubation ( n = 5). ( D ) Protein synthesis in liver organoids treated with or without CB839 and NEAA for 48 h was determined by L-homopropargylglycine (HPG) mean fluorescence intensity (MFI), ( n = 5). (E) Proliferation of liver organoids treated with or without CB839 and NEAA for 3 h was determined by BrdU incorporation, ( n = 5). All data are means ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, one-way ANOVA was used
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    Image Search Results


    GLS1-mediated glutamine metabolism maintains cell proliferation by providing proliferative response substrates. ( A ) Representative bright-field images of liver organoids before (upper panel) and after (lowing panel) the treatment with or without CB839 and NEAA for 48 h (Bar = 1000 μm) and the quantification of organoid size ( B ). ( C ) Cell viability measurement was performed using presto blue. Relative cell viability was calculated by normalizing to Control after 48 h of incubation ( n = 5). ( D ) Protein synthesis in liver organoids treated with or without CB839 and NEAA for 48 h was determined by L-homopropargylglycine (HPG) mean fluorescence intensity (MFI), ( n = 5). (E) Proliferation of liver organoids treated with or without CB839 and NEAA for 3 h was determined by BrdU incorporation, ( n = 5). All data are means ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, one-way ANOVA was used

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: The metabolic shift of glutaminase 2 to glutaminase 1 promotes LGR5 + progenitor cell proliferation in liver cirrhosis

    doi: 10.1007/s00018-025-05772-z

    Figure Lengend Snippet: GLS1-mediated glutamine metabolism maintains cell proliferation by providing proliferative response substrates. ( A ) Representative bright-field images of liver organoids before (upper panel) and after (lowing panel) the treatment with or without CB839 and NEAA for 48 h (Bar = 1000 μm) and the quantification of organoid size ( B ). ( C ) Cell viability measurement was performed using presto blue. Relative cell viability was calculated by normalizing to Control after 48 h of incubation ( n = 5). ( D ) Protein synthesis in liver organoids treated with or without CB839 and NEAA for 48 h was determined by L-homopropargylglycine (HPG) mean fluorescence intensity (MFI), ( n = 5). (E) Proliferation of liver organoids treated with or without CB839 and NEAA for 3 h was determined by BrdU incorporation, ( n = 5). All data are means ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, one-way ANOVA was used

    Article Snippet: Rescue experiments were performed with 5 mM glutathione reduced ethyl ester (GSH-EE), 1x MEM Non-Essential Amino Acids solution (NEAA) from Thermo Fisher Scientific.

    Techniques: Control, Incubation, Fluorescence, BrdU Incorporation Assay

    Schematic model illustrating the effect of the swift of GLS2 to GLS1 on ROS-Wnt/β-catenin signaling and proliferation in liver organoids. GLS1 reduces the generation of ROS, promotes nuclear translocation of β-Catenin and then promotes Wnt/β-Catenin target genes transcription in liver organoids. In addition, GLS1 accelerates the metabolism of Glutamine and provides proliferative response substrates such as amino acids and nucleotides for cell proliferation. Abbreviations: Gln, Glutamine; GSH, Glutathione; Glu, Glutamate; ROS, Reactive oxygen species; NEAA: non-essential amino acids

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: The metabolic shift of glutaminase 2 to glutaminase 1 promotes LGR5 + progenitor cell proliferation in liver cirrhosis

    doi: 10.1007/s00018-025-05772-z

    Figure Lengend Snippet: Schematic model illustrating the effect of the swift of GLS2 to GLS1 on ROS-Wnt/β-catenin signaling and proliferation in liver organoids. GLS1 reduces the generation of ROS, promotes nuclear translocation of β-Catenin and then promotes Wnt/β-Catenin target genes transcription in liver organoids. In addition, GLS1 accelerates the metabolism of Glutamine and provides proliferative response substrates such as amino acids and nucleotides for cell proliferation. Abbreviations: Gln, Glutamine; GSH, Glutathione; Glu, Glutamate; ROS, Reactive oxygen species; NEAA: non-essential amino acids

    Article Snippet: Rescue experiments were performed with 5 mM glutathione reduced ethyl ester (GSH-EE), 1x MEM Non-Essential Amino Acids solution (NEAA) from Thermo Fisher Scientific.

    Techniques: Translocation Assay